Research Technician

Job ID: req2576
Employee Type: nonexempt full-time
Division: Applied & Development Research Program
Facility: Frederick: Ft Detrick
Location: PO Box B, Frederick, MD 21702 USA

The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc. The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.

Accountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way.

PROGRAM DESCRIPTION
The In Vivo Evaluation Group, BTB, DTP is establishing both in vitro and in vivo tumor lines from patient biopsies from DCTD sponsored clinical trials with the goal of developing models for NCI's Patient Derived Models Repository (PDMR). The PDMR is a repository of patient-derived models that include in vivo tumor fragments, in vitro tumor lines (2D and 3D), cancer associated fibroblasts, and DNA/RNA isolates. The goal of the PDMR is to distribute well validated and highly characterized material to researchers worldwide. A secondary mission is to evaluate the efficacy of approved oncology drugs in combination or as single agents in a subset of rare and recalcitrant PDX tumor models.

KEY ROLES/RESPONSIBILITIES
Establish 2D cell cultures from patient samples and from tumors isolated from mice implanted with human tumors
Perform routine cell culture tasks for both 2D and 3D organoid cultures to include cell line maintenance, passaging, and cryopreservation
Prepare large batches of cells (25-150 flask harvests) for in vivo drug studies or for large scale cryopreservation for the repository
Prepare all required medias, components and reagents used in the culture of 2D and 3D organoid cell cultures
Maintain precise records of each patient derived cell line with the intent of delivering carefully documented samples to researchers worldwide.
Aid in other routine tasks as needed such as cell photography, tube/plate labeling, cell deliveries/pick-ups etc

BASIC QUALIFICATIONS
Possession of a BS/BA degree from an accredited college or university (according to CHEA). (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency.
Ability to be flexible to meet changing needs
Organizational skills and database management skills
Ability to work in a fast-paced environment
Ability to work under Biosafety Level-2 conditions
Ability to work alone and as part of a team

Required Skills/Experience:
BASIC QUALIFICATIONS
To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:
Knowledge of Microsoft office programs (Word, Excel, PowerPoint)
Organizational skills and database management skills
Ability to maintain precise records
Good written and verbal communication skills and attention to detail

PREFERRED QUALIFICATIONS
Knowledge of sterile eukaryotic tissue culture techniques
Knowledge of organoid cell culture

JOB HAZARDS OR REQUIREMENTS
Job hazard or requirementEnroll in OSHA BBP. Must be medically cleared to receive any necessary immunizations per protocol requirements.
Job hazard or requirementEmployee would receive vaccinations required and determined by Occupational Health Services (OHS) and/or mandated by Federal Health Standard Regulations
Job hazard or requirement

Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)

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