Post Doctoral Fellow, Zombie HIV Research

Job ID: req2662
Employee Type: exempt full-time
Division: Applied & Development Research Program
Facility: Frederick: Ft Detrick
Location: PO Box B, Frederick, MD 21702 USA

The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc. The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.

Accountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way.

Position Overview:

PROGRAM DESCRIPTION

The Laboratory of Human Retrovirology and Immunoinformatics (LHRI) has collaborated with the National Institute of Allergy and Infectious Diseases (NIAID) and supported their clinical studies for patients infected with HIV, SARS-Cov2 or other viruses. LHRI was the first to demonstrate the potent anti-viral activity of Interleukin-27 (IL-27) and the potent Interferon inducer of Ku70, a DNA repair protein. We also maintain the Database for Annotation, Visualization and Integrated Discovery (DAVID) (https://david.ncifcrf.gov/).

Recently, LHRI initiated a study of the function of defective HIV, Zombie HIV (Proc Natl Acad Sci U S A. 2020;117(7):3704-3710. doi: 10.1073/pnas.1917876117) in the infected cells. We are looking for a passionate scientist who will be dedicated to this challenging project.

KEY ROLES/RESPONSIBILITIES

This Postdoctoral Fellow will be involved in:

• Investigation of the molecular mechanism of virus replication/recombination and the biological function of zombie HIV
• Manipulation of confocal microscopes, fluorescence microscopy, FlowSight imaging flow cytometry, Sanger DNA seq, Nexseq, single-cell RNA-seq, Quanterix ELISA system, RNA scope, and handing Big Data analysis
• Publishing paper(s) and presenting the findings in the international or domestic scientific conference(s) as the first author

BASIC QUALIFICATIONS

To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:

• Possession of a PhD degree from an accredited college or university according to the Council for Higher Education Accreditation (CHEA) or eight (8) years of related experience. Foreign degrees must be evaluated for U.S. equivalency
• No work experience required with a Doctorate degree
• Publication of the author paper(s)
• Ability to obtain and maintain a security clearance

PREFERRED QUALIFICATIONS

Candidates with these desired skills will be given preferential consideration:

• General molecular biology techniques (RNA and DNA extraction, RT-PCR)
• Tissue culture (primary cells and cell lines)
• Transfection of DNA, siRNA or shRNA and Handling of lentiviruses
• Western blotting and ELISA
• Statistical analysis skills

JOB HAZARDS

• This position is subject to working with or have potential for exposure to infectious material, requiring medical clearance and immunizations

Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)

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