Post Doctoral Fellow
October 10, 2023
Post Doctoral Fellow
Job ID: req3600
Employee Type: nonexempt full-time
Division: Applied & Development Research Program
Facility: Frederick: Ft Detrick
Location: PO Box B, Frederick, MD 21702 USA
The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc. The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.
Accountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way.
PROGRAM DESCRIPTION
The Laboratory of Human Retrovirology and Immunoinformatics (LHRI: former LHR), Applied/ Developmental Research Directorate ( https://david.ncifcrf.gov ) has investigated drug resistance in people living with HIV/AIDS (PLWHA) in the National Institute of Allergy and Infectious Diseases (NIAID) clinical therapy since 2001 to define salvage or de novo therapies. LHRI has also studied the function of novel anti-viral cytokines (Interluekine-27 and Interferon Lambda-1) and the innate immune responses for developing novel immunotherapies.
KEY ROLES/RESPONSIBILITIES
This Post Doctoral Fellow will work under the supervision of the lab head, Dr. Tomozumi Imamichi, and study the regulation of HIV infection and replication in primary cells (macrophages, T cells, and dendritic cells). In this position, the candidate will use LHRI-discovered none-coding RNAs, a combination of cytokines, or extracellular vesicles as replication enhancers or suppressors and researches the molecular mechanism of the regulation of the processing of HIV GagPol polyprotein and virus release, utilizing molecular biology and immunological techniques with the state-of-the-art technology, e.g., single-cell (Sc) proteomics, Sc-RNA seq, and NS300. Postdoctoral Fellowship appointments are made for one year, renewable annually, with the manager's approval. We anticipate a period of up to three years for this position.
BASIC QUALIFICATIONS
To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:
PREFERRED QUALIFICATIONS
Candidates with these desired skills will be given preferential consideration:
JOB HAZARDS
Commitment to Diversity
All qualified applicants will receive consideration for employment without regard to sex, race, ethnicity, age, national origin, citizenship, religion, physical or mental disability, medical condition, genetic information, pregnancy, family structure, marital status, ancestry, domestic partner status, sexual orientation, gender identity or expression, veteran or military status, or any other basis prohibited by law. Leidos will also consider for employment qualified applicants with criminal histories consistent with relevant laws.
#readytowork
Job ID: req3600
Employee Type: nonexempt full-time
Division: Applied & Development Research Program
Facility: Frederick: Ft Detrick
Location: PO Box B, Frederick, MD 21702 USA
The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc. The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.
Accountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way.
PROGRAM DESCRIPTION
The Laboratory of Human Retrovirology and Immunoinformatics (LHRI: former LHR), Applied/ Developmental Research Directorate ( https://david.ncifcrf.gov ) has investigated drug resistance in people living with HIV/AIDS (PLWHA) in the National Institute of Allergy and Infectious Diseases (NIAID) clinical therapy since 2001 to define salvage or de novo therapies. LHRI has also studied the function of novel anti-viral cytokines (Interluekine-27 and Interferon Lambda-1) and the innate immune responses for developing novel immunotherapies.
KEY ROLES/RESPONSIBILITIES
This Post Doctoral Fellow will work under the supervision of the lab head, Dr. Tomozumi Imamichi, and study the regulation of HIV infection and replication in primary cells (macrophages, T cells, and dendritic cells). In this position, the candidate will use LHRI-discovered none-coding RNAs, a combination of cytokines, or extracellular vesicles as replication enhancers or suppressors and researches the molecular mechanism of the regulation of the processing of HIV GagPol polyprotein and virus release, utilizing molecular biology and immunological techniques with the state-of-the-art technology, e.g., single-cell (Sc) proteomics, Sc-RNA seq, and NS300. Postdoctoral Fellowship appointments are made for one year, renewable annually, with the manager's approval. We anticipate a period of up to three years for this position.
BASIC QUALIFICATIONS
To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:
- Possession of a Ph.D., MD, or DVM degree with an emphasis in virology or molecular biology from an accredited college or university according to the Council for Higher Education Accreditation (CHEA). (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency
- Standard molecular and cellular biological skills (qRT-PCR , WB , ELISA, cell culture, etc.)
- Ability to obtain and maintain a security clearance
PREFERRED QUALIFICATIONS
Candidates with these desired skills will be given preferential consideration:
- Experience handling of replication competent viruses.
- Isolation of extracellular vesicles or lentivirus particles
- Biological study of plasma membrane, and characterization of lipid/cholesterol
- Cell-to-cell fusion assay
- Confocal microscopic assay using Zeiss LSM 800 with Airyscan.
- IP, FISH, IHC
- FACS data analysis using the FlowJoe
JOB HAZARDS
- This position is subject to working with or have potential for exposure to infectious material, requiring medical clearance and immunization
Commitment to Diversity
All qualified applicants will receive consideration for employment without regard to sex, race, ethnicity, age, national origin, citizenship, religion, physical or mental disability, medical condition, genetic information, pregnancy, family structure, marital status, ancestry, domestic partner status, sexual orientation, gender identity or expression, veteran or military status, or any other basis prohibited by law. Leidos will also consider for employment qualified applicants with criminal histories consistent with relevant laws.
#readytowork