Scientist I- Electron Microscopy

September 10, 2023
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Scientist I- Electron Microscopy

Job ID: req3573
Employee Type: exempt full-time
Division: Cancer Research Technology Program
Facility: Frederick: ATRF
Location: 8560 Progress Dr, Frederick, MD 21701 USA

The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc. The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.

Accountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way.

PROGRAM DESCRIPTION

Vaccine Research Center Electron Microscopy Unit (VRC-EMU), located at National Cancer Institute (NCI) in Frederick, MD, performs electron microscopy analysis of proteins and related specimens for the Vaccine Research Center (VRC) of National Institute of Allergy and Infectious Diseases (NIAID). Cryo-EM, negative-stain EM, and classical thin-section EM are all being used to address a wide spectrum of questions broadly related to vaccine research and development. High-throughput cryo-EM data collection and high-resolution single particle analysis of cryo-EM data by VRC-EMU are central to structure-based vaccine design efforts at VRC NIAID. VRC-EMU runs a Thermo Scientific Titan Krios and a Thermo Scientific Talos F200C electron microscopes and shares access to multiple smaller Thermo Scientific and Hitachi electron microscopes.

KEY ROLES/RESPONSIBILITIES
  • Screening of protein samples using negative-stain electron microscopy
  • Specimen preparation and screening for high-resolution structural analysis by cryo-EM
  • Cryo-EM data collection on Titan Krios and other electron microscopes
  • Computational processing of cryo-EM and negative-stain EM data

BASIC QUALIFICATIONS
  • Possession of a Ph.D. in Biochemistry, Biophysics, or a field related to Biomedical Research from an accredited college/university according to the Council for Higher Education Accreditation (CHEA). Foreign degrees must be evaluated for U.S. equivalency
  • Experience with protein specimen preparation and screening using cryo-EM and negative-stain EM
  • Experience with data collection using electron microscopes

PREFERRED QUALIFICATIONS
  • Extensive experience with protein screening by negative-stain EM and preparation and optimization of cryo-EM specimens
  • Extensive experience with automatic high-throughput cryo-EM data collection on high-end electron microscopes equipped with autoloaders
  • Experience with solving structures of proteins, protein complexes or virus-like particles using single particle analysis of cryo-EM data


Commitment to Diversity
All qualified applicants will receive consideration for employment without regard to sex, race, ethnicity, age, national origin, citizenship, religion, physical or mental disability, medical condition, genetic information, pregnancy, family structure, marital status, ancestry, domestic partner status, sexual orientation, gender identity or expression, veteran or military status, or any other basis prohibited by law. Leidos will also consider for employment qualified applicants with criminal histories consistent with relevant laws.

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